Sandwich ELISA of human plasma using the recombinant Human IgA antibody as the capture (100ng/well), and biotinylated anti-human light chains ( kappa;+ lambda;) antibody, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human IgA using the recombinant Human IgA antibody as the capture (100ng/well), and biotinylated anti-human light chains ( kappa;+ lambda;) antibody, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of human immunoglobulins shows the recombinant Human IgA antibody reacts to both IgA1 & IgA2. No cross reactivity with IgG, IgM, IgD, or IgE.
ELISA Titration: the plate was coated with different amounts of human IgA. A serial dilution of the recombinant Human IgA antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Supplier Page from NSJ Bioreagents for Recombinant Human IgA Antibody (A1 & A2) [Recombinant Rabbit Monoclonal]